The buffy coat method: a tool for detection of blood parasites without staining procedures

Evaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia.

The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram-positive bacteria belonging to six species and Gram-negative bacteria from 10 species were isolated and identified by ...

Peripheral blood buffy coat smear : A promising tool for diagnosis of visceral

11 Confirmative diagnosis of Visceral Leishmaniasis (VL) is still being a challenge at the primary 12 health care facilities in most of the rural endemic areas in the Indian sub-continent. Conventional 13 methods for parasitological confirmation are risky and require skilled personnel hence, 14 unreachable to the poor people in the endemic region. Buffy coat smear microscopy, as a 15 minimally ...

Peripheral blood buffy coat smear: a promising tool for diagnosis of visceral leishmaniasis.

Confirmative diagnosis of visceral leishmaniasis (VL) is still a challenge at the primary health care facilities in most of the rural areas of endemicity in the Indian subcontinent. Conventional methods for parasitological confirmation are risky and require skilled personnel, and hence they are unavailable to the poor people in the regions of endemicity. Buffy coat smear microscopy, as a minima...

Still Another Method of Staining Malaria Parasites

Use of the Quantitative Buffy Coat system for detection of parasitemia in patients with babesiosis.

Quantitative Buffy Coat analysis and blood smears were performed on a total of 47 blood samples. The technique showed 100% correlation with the blood smears in 9 samples containing babesia and 10 samples containing malaria, with some differential features distinguishing the two infections. Quantitative Buffy Coat analysis provides a simple and rapid method for the detection of parasitemia in ca...